United States Patent | 5,310,669 |
Richmond, et. al. | May 10, 1994 |
Inventors: | Richmond; Robert C. (Etna, NH); Gibson; Ursula J. (West Lebanon, NH). |
Assignee: | The Trustees of Dartmouth College (Hanover, NH). |
Appl. No.: | 901,911 |
Filed: | Jun. 22, 1992 |
Intl. Cl.: | C12M 3/00; C12N 5/00; C12N 11/00; C12N 11/02; |
U.S. Cl.: | 435/177.; 435/174.; |
Field of Search: | 174;177;180;181;240.1;240.2;240.23;240.241;240.243;240.242;284;285 |
4,363,634 | Dec., 1982 | Schall, Jr. | 435/181 |
4,438,196 | Mar., 1984 | Lantero, Jr. | 435/181 |
5,108,923 | Apr., 1992 | Benedict et al. | 435/240 .23 |
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Substrates having a surface coated with fullerene and a substance attached thereto are disclosed. Cell culture substrates having a fullerene-coated surface are useful in methods of growing cells on the fullerene-coated surface. Methods of preparing cell culture substrates for cell attachment and growth by coating a surface with fullerene are provided. Cells can be grown on a fullerene-coated surface in the presence of a substance such as a cytokine, growth hormone or a drug, to evaluate the interaction between the substance and the cells. Methods for increasing cell membrane premability and for introducing a substance, such as a DNA or RNA vector, into a cell are also provided.